Laboratory for Future Interdisciplinary Research of Science and Technology, Institute of Innovative Research, Tokyo Institute of Technology Nisisako Group Microfluidic Interface Lab.

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Research Topics

Below are some examples of the research topics in our lab.

Understanding Droplet Breakup in a Micropost Array NEW

Understanding Droplet Breakup in a Micropost Array

Recent studies have highlighted the capability of generating relatively uniform (quasi-monodisperse) droplets by directing the flow of two immiscible liquids (e.g., water and oil) through an array of aligned microscopic posts. Our research has unveiled two distinct breakup modes contingent upon the shear force magnitude. Furthermore, it has been elucidated that the diameter of the resulting droplets exhibits a power-law correlation with Capillary number.

Selected Papers

  1. Masui et al., Lab Chip 23, 4959–4966(2023).

Particle Separation

Microfluidic Particle Separation

Particle separation is a crucial necessity across diverse fields, including analytical chemistry and material production. Our research group is studying a microfluidic technology that allows for highly efficient separation of particles through periodically arrayed microposts. Our accomplishments include the successful separation of polymer particles, droplets, cells, and more.

Selected Papers

  1. Tottori et al., RSC Adv. 7, 35516 (2017).
  2. Tottori et al., Biomicrofluidics 10, 014125 (2016).

Microlenses Fabrication


We are pioneering a novel approach to fabricating microlenses, bypassing the need for a solid mold through droplet microfluidics. This technique involves the creation of multiphase droplets wherein a curable and non-curing liquids undergo phase separation. By subsequent photopolymerization, microlenses exhibiting diverse shapses (biconvex, biconcave, concave and convex) can be produced.

Selected Papers

  1. Nisisako et al., Micromachines 6, 1435–1444 (2015).
  2. Nisisako et al., Small 10, 5116–5125 (2014).
  3. Nisisako et al., 精密工学会誌 79, 460–466 (2013)

Artificial Lipid Bilayer Platform


Lipid bilayer membranes serve as fundamental components in cell membranes. In recent years, systems utilizing artificial lipid bilayer membranes have found extensive applications in diverse areas such as electrophiological tests, biosensors, and artificial cell studies. We have developed a highly efficient method for fabricating planar lipid bilayers within microchannles or microchambers, facilitating rapid measurements of the in-vitro passsive membrane permeability of potential drug candidates.

Selected Papers

  1. Nisisako et al., Analyst 138, 6793-6800 (2013).

Parallelization for Scaled-Up Production


We are conducting research on technology to scale up the production of various droplets and particles by parallelizing (numbering up) a large number of microchannels at high density. To date, we have parallelized tens to hundreds of microchannels on a chip of several centimeters square to increase the production rate of single-phase emulsion droplets, Janus droplets, multi-phase emulsions, and various fine particles with excellent size uniformity. We are conducting research aimed at further scaling up.

Selected Papers

  1. Nisisako et al., Lab Chip 12, 3426–3435 (2012).
  2. Nisisako et al., Lab Chip 8, 287–293 (2008).